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Overview

SPE is a method of sample preparation that concentrates and purifies analytes from solution by sorption onto a sorbent contained in either a disposable solid phase cartridge or a 96-well plate, followed by elution of the analyte with a solvent and reconstitution into a mobile phase that is appropriate for the instrumental method used for analysis.

 

Classification 

According to principle of reaction, SPE is divided into the following three categories.

1. Normal-phase extraction- Adsorbents used in normal phase extraction are polar. 

2. Reversed-phase extraction- The sorbent and the target analytes by reversed-phase extraction is usually the non-polar or weak polar, relying mainly on non polar and non polar interactions, is a fan of Edward force or dispersion force.

3. Ion exchange extraction- is relying on the interaction between target analytes and sorbent.

 

Selection

Select proper sorbent according to the difference between target analytes and disruptors such as polar, molecular weight and pKa, etc.

Sample

Dissolve in organic solvent

Methanol or Methanol/Water

Reversed Phase Sorbent

CN,C18,Phenyl

Normal Phase Sorbent

(Bonding Force)

NH2, COOH, Diol

Hexane

Normal Phase Sorbent

(Bonding Force)

NH2, COOH, Diol

Normal Phase Sorbent

(Adsorption Force)

Silica, Florisil

Dissolve in water

Ion Type

Cation

SCX

Anion

SAX

Non ion Type

Reversed Phase Sorbent

C18,C8,C2,Phenyl

Reversed Phase Sorbent

C18,C8,C2,Phenyl


For reverse phase, normal phase and adsorption type SPE cartridges, the quantity of target analytes to SPE sorbent ratio is generally not more than 5%. For ion exchange SPE cartridges, need to consider the ion exchange capacity.  Provide the conventional ion exchange capacity of Starlab is about 0.3meq/g, the following table for SPE cartridges capacity parameters:


Specification

Maximum Sample Load

Volume

Minimum Elution Volume

50-100mg

2.5mg-5mg

1mL

100-200μL

100-200mg

5mg-10mg

3mL

200-500μL

200mg-1g

10mg-50mg

6mL

500μL-6mL

500mg-2g

25mg-100mg

12mL

3mL-10mL


Starlab SPE Cartridges are made of medical grade PP and forming in one time. Frits are made of UHMW-PE. There is a variety of models to choose. Starlab provides SPE OEM service in order to support you to establish your own SPE brands easily.

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