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The difference of PVDF membrane and nylon membrane
Date: 2016-09-22

Brief introduction
PVDF membrane

Polyvinylidene fluoride(PVDF) membrane is a solid  purport of Western blotting commonly used  in western blot method . It is  hydrophobic and owns fast flow rate and very low protein binding. Generally compatible with most common  solvents. One of the good choice for protein recovery applications.

 

Nylon66/Polyamide (NY)
 Nylon 66 is a kind of  hydrophilic membrane and resistant to a range of organic solvents but not suitable for strong acid.  The nylon membrane is suitable for use with high PH samples

It is a kind of synthesis of long chain polyamide membrane, combination of protein and nucleic acid has a strong ability, it also ,can take the place of cellulose nitrate film used in molecular imprinting and hybridization experiment.

 

Difference
1.combining capacity
PVDF membrane in combination with DNA and RNA capacity can reach 125-125 mu g/cm2
Nylon membrane in combination with DNA and RNA the capacity can reach 480-600 mu g/cm2, it can be combined with as little as 10 bp DNA fragment;

2.thermal adaptability

The PVDF membrane combined with strong, high temperature resistance. It is especially suitable for protein imprinting. The nucleic acid of pyrimidine bases can be combined with the positive charge after teh membrane baking or ultraviolet light .

 

3. repeatability
The PVDF membrane can be repeated used because the high repeatability of itself.

Nylon membrane can be repeatedly used in molecular hybridization, after hybridization, the probe of molecules be eluted by alkali denaturation .

Starlab Scientific Co., Ltd
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