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What’s the HPLC?
Date: 2017-04-11

High performance liquid chromatography (HPLC) is an important branch of chromatography. Liquid-based mobile phase is used to pump a mobile solvent such as a single solvent with different polarities or different proportions of mixed solvents, buffers and the like into a stationary phase Column, after the separation of the components in the column, into the detector for testing, in order to achieve the analysis of the sample. The method has become an important separation and analysis technology in the fields of chemistry, medicine, industry, agriculture, commodity inspection and forensic examination.


 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

HPLC with "three high one wide one fast" features:

1.high pressure: the mobile phase is liquid, through the column, the resistance is greater, in order to quickly through the column, the carrier must be high pressure.

2.high separation efficiency: The stationary phase and the mobile phase can be selected to achieve the best separation effect, many times higher than the separation efficiency of industrial distillation columns and gas chromatography.

3.high sensitivity: UV detector up to 0.01ng, the amount of injection in the order of μL.

4.wide range of applications: more than 70 percent of the organic compounds can be analyzed by high performance liquid chromatography, especially high boiling point, macromolecules, strong polarity, poor thermal stability of the separation analysis, showing the advantage.

5.fast analysis speed: The analysis speed more faster than the classic liquid chromatography, usually analyze a sample in 15 to 30 minutes, some samples even in 5 minutes to complete, generally less than 1 hour.

http://www.star-labsci.com/hplc-column/